Honours

• Won 10x Genomics Single Cell India Grant program, 2023
• ICMR Ad-Hoc Research Grant, 2023
• GSBTM cancer research grant, 2021
• DST-SERB Start-up Research Grant (SRG) 2020
• International PhD grant for foreign nationals by the Italian government and CARIPARO
• CSIR diamond jubilee research interns award
• UGC-CSIR NET
• MSc Biotechnology Scholarship from the Department of Biotechnology (DBT)

Publications

• Pandey N, Sharma M, Mathur A, Nzelu GA, Hakimullah M, Jha IP, Chandra O, Mishra S, Sharma A, Foo R, Mandoli A, DasGupta R, Kumar V. Deciphering the phenotypic heterogeneity and drug response in cancer cells using genome-wide activity and interaction of chromatin domains bioRxiv 2023.01.15.524115; doi: https://doi.org/10.1101/2023.01.15.524115
• Parshuram Satpute D, Shirwadkar U, Kumar Tharalla A, Dattatray Shinde S, Nikhil Vaidya G, Joshi S, Patel Vatsa P, Jain A, Singh AA, Garg R, Mandoli A,, Kumar D. Discovery of fluorinated 2‑Styryl 4(3H)-quinazolinone as potential therapeutic hit for oral cancer. Bioorg Med Chem. 2023 Mar 1;81:117193. doi: 10.1016/j.bmc.2023.117193
• Ambika Chamoli, Abhishek S.Gosavi, Urjita P.Shirwadkar, Khushal V.Wangdale, Santosh Kumar Behera, Nawneet Kumar Kurrey, Kiran Kalia,Mandoli A. (2021). Overview of oral cavity squamous cell carcinoma: Risk factors, mechanisms, and diagnostics. Oral Oncology . 121, 105451. doi.org/10.1016/j.oraloncology.2021.105451
• Nargund AM, Xu C,Mandoli A,, Okabe A, Chen GB, Huang KK, Sheng T, Yao X, Teo JMN, Sundar R, Kok YJ, See YX, Xing M, Li Z, Yong CH, Anand A, A I ZF, Poon LF, Ng MSW, Koh JYP, Ooi WF, Tay ST, Ong X, Tan ALK, Grabsch HI, Fullwood MJ, Teh TB, Bi X, Kaneda A, Li S, Tan P. Chromatin Rewiring by Mismatch Repair Protein MSH2 Alters Cell Adhesion Pathways and Sensitivity to BET Inhibition in Gastric Cancer. Cancer Res . 2022 Jul 18;82(14):2538-2551. doi: 10.1158/0008-5472.CAN-21-2072
• Davies R, Liu L, Taotao S, Tuano N, Chaturvedi R, Huang KK, Itman C, Mandoli A,, Qamra A, Hu C, Powell D, Daly RJ, Tan P, Rosenbluh J. CRISPRi enables isoform-specific loss-of-function screens and identification of gastric cancer-specific isoform dependencies. Genome Biology. 2021 Jan 26;22(1):47. doi: 10.1186/s13059-021-02266-6
• van Bergen MGJM, van Oorschot R, Bergevoet SM, de Graaf AO, Tönnissen ELRTM, Stevens-Linders E, Neveling K, Jansen PWTC, Baltissen MPA, Vermeulen M,Mandoli A,, Martens JHA, Preijers F, Jansen JH, van der Reijden BA. Characterization of a genomic region 8 kb downstream of GFI1B associated with myeloproliferative neoplasms. Biochim Biophys Acta Mol Basis Dis. 2021 Dec 1;1867(12):166259. doi: 10.1016/j.bbadis.2021.166259
• Cordonnier G,Mandoli A,, Cagnard N, et al. CBFβ-SMMHC Affects Genome-wide Polycomb Repressive Complex 1 Activity in Acute Myeloid Leukemia. Cell Rep. 2020;30(2):299–307.e3
• Tijchon E, Yi G,Mandoli A,, et al. The acute myeloid leukemia associated AML1-ETO fusion protein alters the transcriptome and cellular progression in a single-oncogene expressing in vitro induced pluripotent stem cellbased granulocyte differentiation model. PLoS One. 2019;14(12):e0226435
• Ooi WF, Nargund,Mandoli A,, Lim KJ, et al. Integrated paired-end enhancer profiling and whole-genome sequencing reveals recurrent CCNE1 and IGF2 enhancer hijacking in primary gastric adenocarcinoma. Gut. 2019;gutjnl-2018-317612
• Yi G,Mandoli A, Jussen L, et al. CBFβ-MYH11 interferes with megakaryocyte differentiation via modulating a gene program that includes GATA2 and KLF1. Blood Cancer J. 2019;9(3):33
• Yi G, Wierenga ATJ, Petraglia F, et al. Chromatin-Based Classification of Genetically Heterogeneous AMLs into Two Distinct Subtypes with Diverse Stemness Phenotypes. Cell Rep. 2019;26(4):1059–1069.e6
• Teo K, Gómez-Cuadrado L, Tenhagen M, Byron A, Rätze M, van Amersfoort M, Renes J, Strengman E, Mandoli A,, Singh AA, Martens JH, Stunnenberg HG, van Diest PJ, Brunton VG, Derksen PWB. E-cadherin loss induces targetable autocrine activation of growth factor signalling in lobular breast cancer. Sci Rep. 2018 Oct 18;8(1):15454. doi: 10.1038/s41598-018-33525-5
• Singh AA, Petraglia F, Nebbioso A, Yi G, Conte M, Valente S, Mandoli A,, Scisciola L, Lindeboom R, Kerstens H, Janssen-Megens EM, Pourfarzad F, Habibi E, Berentsen K, Kim B, Logie C, Heath S, Wierenga ATJ, Clarke L, Flicek P, Jansen JH, Kuijpers T, Yaspo ML, Valle VD, Bernard O, Gut I, Vellenga E, Stunnenberg HG, Mai A, Altucci L, Martens JHA. Multi-omics profiling reveals a distinctive epigenome signature for high-risk acute promyelocytic leukemia. Oncotarget. 2018 May 22;9(39):25647-25660. doi: 10.18632/oncotarget.25429
• Petraglia F, Singh AA, Carafa V, Nebbioso A, Conte M, Scisciola L, Valente S, Baldi A, Mandoli A, Petrizzi VB, Ingenito C, De Falco S, Cicatiello V, Apicella I, Janssen-Megens EM, Kim B, Yi G, Logie C, Heath S, Ruvo M, Wierenga ATJ, Flicek P, Yaspo ML, Della Valle V, Bernard O, Tomassi S, Novellino E, Feoli A, Sbardella G, Gut I, Vellenga E, Stunnenberg HG, Mai A, Martens JHA, Altucci L. Combined HAT/EZH2 modulation leads to cancer-selective cell death. Oncotarget. 2018 May 22;9(39):25630-25646. doi: 10.18632/oncotarget.25428
• Marneth AE, Botezatu L, Hönes JM, Israël JCL, Schütte J, Vassen L, Lams RF, Bergevoet SM, Groothuis L, Mandoli A,, Martens JHA, Huls G, Jansen JH, Dührsen U, Berg T, Möröy T, Wichmann C, Lo MC, Zhang DE, van der Reijden BA, Khandanpour C. GFI1 is required for RUNX1/ETO positive acute myeloid leukemia. Haematologica. 2018 Sep;103(9):e395-e399. doi: 10.3324/haematol.2017.180844
• Iacono G, Dubos A, Méziane H, Benevento M, Habibi E, Mandoli A,, Riet F, Selloum M, Feil R, Zhou H, Kleefstra T, Kasri NN, van Bokhoven H, Herault Y, Stunnenberg HG. Increased H3K9 methylation and impaired expression of Protocadherins are associated with the cognitive dysfunctions of the Kleefstra syndrome. Nucleic Acids Res. 2018 Jun 1;46(10):4950-4965. doi: 10.1093/nar/gky196
• Cordonnier G, Mandoli A, Radhouane A, et al. CBFβ-SMMHC regulates ribosomal gene transcription and alters ribosome biogenesis. Leukemia. 2017;31(6):1443–1446
• Prange KHM,Mandoli A, Kuznetsova T, et al. MLL-AF9 and MLL-AF4 oncofusion proteins bind a distinct enhancer repertoire and target the RUNX1 program in 11q23 acute myeloid leukemia. Oncogene. 2017;36(23):3346–3356
• Singh AA,Mandoli A, Prange KH, Laakso M, Martens JH. AML associated oncofusion proteins PML-RARA, AML1-ETO and CBFB-MYH11 target RUNX/ETS-factor binding sites to modulate H3ac levels and drive leukemogenesis. Oncotarget .2017;8(8):12855–12865
• Astle WJ, et al. The Allelic Landscape of Human Blood Cell Trait Variation and Links to Common Complex Disease. Cell. 2016 Nov 17;167(5):1415-1429.e19. doi: 10.1016/j.cell.2016.10.042
• Chen L, et al. Epigenetic and Transcriptional Variation in Human Immune Cells.Cell. 2016 Nov 17;167(5):1398-1414.e24. doi: 10.1016/j.cell.2016.10.026
• Mandoli A,, Singh AA, Prange KHM, et al. The Hematopoietic Transcription Factors RUNX1 and ERG Prevent AML1-ETO Oncogene Overexpression and Onset of the Apoptosis Program in t(8;21) AMLs. Cell Rep. 2016;17(8):2087–2100
• Vegi NM, Klappacher J, Oswald F, Mulaw MA, Mandoli A,, Thiel VN, Bamezai S, Feder K, Martens JHA, Rawat VPS, Mandal T, Quintanilla-Martinez L, Spiekermann K, Hiddemann W, Döhner K, Döhner H, Stunnenberg HG, Feuring-Buske M, Buske C. MEIS2 Is an Oncogenic Partner in AML1-ETO-Positive AML. Cell Rep. 2016 Jul 12;16(2):498-507. doi: 10.1016/j.celrep.2016.05.094
• Mandoli A,, Prange K, Martens JH. Genome-wide binding of transcription factors in inv(16) acute myeloid leukemia. Genom Data. 2014 Jun 20;2:170-2. doi: 10.1016/j.gdata.2014.06.014
• Sotoca AM, Prange KH, Reijnders B,Mandoli A,, Nguyen LN, Stunnenberg HG, Martens JH. The oncofusion protein FUS-ERG targets key hematopoietic regulators and modulates the all-trans retinoic acid signaling pathway in t(16;21) acute myeloid leukemia. Oncogene. 2016 Apr 14;35(15):1965-76. doi: 10.1038/onc.2015.261
• Saeed S,et al. Epigenetic programming of monocyte-to-macrophage differentiation and trained innate immunity. Science. 2014 Sep 26;345(6204):1251086. doi: 10.1126/science.1251086
• De Bellis F, et al. Context-selective death of acute myeloid leukemia cells triggered by the novel hybrid retinoid-HDAC inhibitor MC2392. Cancer Res. 2014 Apr 15;74(8):2328-39. doi: 10.1158/0008-5472.CAN-13-2568
• Mandoli A,, Singh AA, Jansen PW, Wierenga AT, Riahi H, Franci G, Prange K, Saeed S, Vellenga E, Vermeulen M, Stunnenberg HG, Martens JH. CBFB-MYH11/RUNX1 together with a compendium of hematopoietic regulators, chromatin modifiers and basal transcription factors occupies self-renewal genes in inv(16) acute myeloid leukemia. Leukemia. 2014 Apr;28(4):770-8. doi: 10.1038/leu.2013.257

Research Scholars

Student Details

Vinal Upadhyay, PhD

Research Associate

Ambika Chamoli

PhD Scholar

Priyanka Vats

PhD Scholar

U.Sai Swetha

PhD Scholar

Expertise

• Hematopoesis, Hematopoietic stem cells, Hematopoietic cancer (Leukemia), Oral squamous cell carcinoma
• Next-generation sequencing (NGS) assays, Genomics, Functional Genomics, Epigenetics (ChIP-seq), Chromatin interactions (HiC, 4C, Hi-ChIP), Transcriptomics (RNA-seq), Gene regulation
• CRISPR-Cas9 genome editing assays, Viral delivery
• Mass-spec based high-throughput proteomics
• Development of the novel in vitro cancer model systems
• Drug repurposing and screening